A simple dot blot assay to measure hygromycin B phosphotransferase activity in whole cell extracts of Neurospora crassa
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چکیده
منابع مشابه
Quantitative immuno-slot blot assay to measure ACMPK protein in cell extracts of Aspergillus nidulans.
A primary means of detecting and identifying specific proteins in cellular extracts is by Western blot analysis (4,13). Quantitation of a protein of interest is generally accomplished using an enzyme-linked immunosorbent assay (ELISA; References 8, 9 and 11) or radioimmunoassay (4,8). When ELISA or radioimmunoassay procedures for the protein of interest are unavailable, densitometric analysis o...
متن کاملTransaminase Activity in Neurospora crassa
It is now believed that the amino groups of isoleucine and valine are accepted by their respective carbon chains during biosynthesis via a transamination. Most of the evidence for this hypothesis has been derived from experiments on isoleucineless and valineless mutants of Escherichia coli and Neurospora crassu (l-4). It is not positively established whether this transamination step can be medi...
متن کاملLarge-scale purification and acute toxicity of hygromycin B phosphotransferase.
OBJECTIVE To provide the acute toxicity data of hygromycin B phosphotransferase (HPT) using recombinant protein purified from E. coli. METHODS Recombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bacterial control plasmid was constructed, and bacteria control protein was extracted and prepared ...
متن کاملDevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
متن کاملCell Fusion in Neurospora crassa
The primary research aims of this thesis were the identification of novel cell fusion mutants of Neurospora crassa and the subsequent functional characterization of selected candidate proteins during conidial anastomosis tube (CAT)-mediated cell fusion by means of genetic, molecular, biochemical and live-cell imaging analysis. Chapter 1 provides a general introduction of the model organism and ...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1995
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1337